The 2024 Kinetics Workshop has been scheduled for January 9-12, 2024, at the Hyatt Regency in Austin, Texas USA. Registration now. Seats are limited.
Skyline of Austin showing the Hyatt Regency Hotel on Lady Bird Lake with 10 miles of hiking trails. Exercise your mind and body at the 2024 Kinetics Workshop. Click on image for more information and to register.
The last workshop in Austin in January 2020 was a great success. Participants came away eager to get back to their labs to implement all that they had learned in the workshop.
Kinetics Workshop Testimonials:
Thank you for organizing this intensive workshop with such passion and desire to share the knowledge you’ve acquired over the years. I’ll go back to the lab with a better and clearer understanding of how to treat my data, which is just a wonderful and valuable gift. Erika Lopez-Alfonzo, University of California Berkeley
This workshop has been transformative in how I view experimental planning and analysis. It has been a great pleasure to attend. Michael Cory, University of Pennsylvania
It has been an absolute pleasure learning enzymology/kinetics from you. Your method of teaching is so refreshing and will truly be helpful in my career. I am excited to pass along knowledge gained from the workshop to my colleagues at MD Anderson where it will be used in all aspects of drug discovery and hopefully impact patient’s lives. Christian Rodriguez, MD Anderson
KinTek Corp: 38 Years of Innovation and Counting
KinTek Corporation is the world leader in the design, manufacture and sales of Stopped-Flow and Quench-Flow instruments and in the development of dynamic software for the kinetic analysis of chemical and biochemical reactions on the millisecond timescale. We help you solve the most complex problems elegantly and simply.
KinTek offers everything you need for direct kinetic and equilibrium measurements using the planet's best instruments and innovative software for data analysis, and now the our founder, Dr. Kenneth Johnson has written a book describing new methods to teach and learn kinetics and rigorously fit data using computer simulation. “Kinetic Analysis for the New Enzymology” is available for purchase exclusively on the KinTek website, below.
In Kinetic Analysis for the New Enzymology, Dr. Johnson brings 40 years of research and sets forth a new approach to teach the conceptual basis underlying kinetic analysis, going beyond the steady-state to directly observe reactions occurring at the active sites of enzymes. Using the full power of computer simulation to model kinetics and equilibria, we escape the bounds of equation-based data fitting and find answers to important mechanistic questions.
The text is built around KinTek Explorer software. Through practice exercises, you will learn how to use dynamic computer simulation to explore key kinetic concepts. Using the software, you can easily scroll the value of a given rate constant, starting concentration of reactants, or output scaling factors, and immediately see the effect on the output signal. This process provides a critical visual link between a model, underlying parameters, and the observable signal.
READ CHAPTER ONE
KinTek Explorer Software
The KinTek Explorer™ is a powerful software package that changes the way you design and interpret experiments. Representing a paradigm shift in data fitting, it takes you past all the simplifying assumptions normally used in fitting kinetic data to extract mechanistic information.
Conventional data fitting requires simplifying approximations to allow mathematical solutions of equations describing a chosen model. This is followed by error-prone fitting of the data to analytical functions, replotting of observed rates versus concentration of reactants and, finally, attempts to extract rate constants using another set of simplified equations derived according to the assumed model. With the release of the KinTek Explorer, conventional methods of data fitting are obsolete!
Data are fit directly to the model based on numerical integration of the rate equations, derived by the software from a simple text description of the model entered by the user. Multiple experiments can be fit simultaneously based on a single, unifying model! Click here for more information.
Graduate student Jiawen Li analyzes her kinetic data to define the rate of pyrophosphate release from the Hepatitis C viral RNA polymerase.
Stopped-Flow Technology
An experiment using a stopped-flow instrument is quite simple in principle. This instrument forces solutions contained in separate drive syringes through a mixing device and into a flow cell. The flow then abruptly stops and a computer is triggered to record data as the freshly mixed solution ages. Although older-style stopped-flow instruments employ air cylinders and stop syringes, KinTek uses numerically controlled servo motors for precise and reproducible rates of mixing. All KinTek stopped-flow models use this elegant and reliable syringe drive system affording a dead time of less than 1 ms.
Our instruments are better than the cheaper air driven models, but our computer controlled servo motors provide improved reliability, ease of use, adaptability, and sample economy. You get cleaner results in less time and with fewer materials.
Auto SF-120
The Auto SF-120 offers outstanding sample economy, requiring only 120 microliters to load a new sample and collect data. The samples are automatically loaded using our proprietary computer-controlled syringe drive and syringe load valve. Simply pipette your sample into a small sample cup and press "Load". The automated loading and extraordinary sample economy allow you to collect more data in less time and using less of your precious samples. An optional robot allows unattended sample loading and data collection. Choose the Auto SF-120 if you need small sample volumes and/or have a large numbers of samples to test. More information...
SF-300x
The SF-300X requires only 300 microliters to load a new sample, second only to the Auto SF-120 in sample economy. What distinguishes the SF-300x is the ability to do efficient double mixing experiments and to add an equilibrium titration module. Choose the SF-300x if you need these capabilities or need to adapt the Stopped-flow to new detection methods. More information....
Chemical Quench-Flow Technology
Stopped-flow techniques are limited to the observation of an available optical signal for the reaction of interest and the optical signals cannot be interpreted rigorously if the extinction coefficients of intermediates or products are not known. Chemical quench-flow methods afford direct measurement of the conversion of substrates to products. By simultaneously fitting stopped-flow and chemical-quench-flow data using KinTek Explorer software, you can derive a single unique model to define the reaction mechanism. For example, see Dr. Johnson's work on HIV reverse transcriptase.
RQF-3 Rapid Chemical Quench Flow
The RQF-3 enables the mixing and quenching of reactants for times as short as 2 milliseconds, using reaction volumes as small as 15 microliters. Double Delay mixing is a standard feature. The RQF-3 also allows optional accessories for Rapid Freeze-Quench and Rapid Photolysis after mixing. Up to three mixing events can be performed. More information....