28 Years of Innovation and Counting
KinTek Corporation is the world leader in the design, manufacture and sales of Stopped-Flow and Quench-Flow instruments and in the development of dynamic software for the kinetic analysis of chemical and biochemical reactions on the millisecond timescale.
KinTek offers everything you need for direct kinetic and equilibrium measurements using the planet's best instruments and innovative software for data analysis. KinTek was founded by Dr. Kenneth Johnson in response to demand from the scientific community for instruments that outperformed those offered by other manufacturers. We continue that principle today of offering the very best instruments and software for kinetic analysis.
We help you solve the most complex problems elegantly and simply.
Education: New Enzymology Kinetics Workshop 2020
Registration is open for the annual New Enzymology Kinetics Workshop given by KinTek founder Dr. Kenneth Johnson.
Recently named a 2019 Biophysical Society Fellow for his “impactful studies using advanced enzyme kinetics in a wide variety of systems”, Dr. Johnson will share his insights, developed over decades of research. The goal of the workshop is for attendees to develop a sound background and intuitive understanding of the information content of kinetic data, and to apply that knowledge to design and rigorously interpret the right experiments to address mechanistic questions most directly.
A central feature of the 4-day intensive workshop is the use of KinTek Explorer software to visualize and explore kinetic models interactively and to rigorously use them to fit data. Tutorials on the use of computer simulation will help develop an intuitive understanding of reaction kinetics, with hands-on problem solving to facilitate integration and mastery of the course material.
Visit https://www.kintekexplorer.com/workshop for full program details and schedule, and to register online. Space is limited and early-registration discounts apply.
KinTek Explorer Software
The KinTek Explorer™ is a powerful software package that changes the way you design and interpret experiments. Representing a paradigm shift in data fitting, it takes you past all the simplifying assumptions normally used in fitting kinetic data to extract mechanistic information.
Conventional data fitting requires simplifying approximations to allow mathematical solutions of equations describing a chosen model. This is followed by error-prone fitting of the data to analytical functions, replotting of observed rates versus concentration of reactants and, finally, attempts to extract rate constants using another set of simplified equations derived according to the assumed model. With the release of the KinTek Explorer, conventional methods of data fitting are obsolete!
Data are fit directly to the model based on numerical integration of the rate equations, derived by the software from a simple text description of the model entered by the user. Multiple experiments can be fit simultaneously based on a single, unifying model! Click here for more information.
An experiment using a stopped-flow instrument is quite simple in principle. This instrument forces solutions contained in separate drive syringes through a mixing device and into a flow cell. The flow then abruptly stops and a computer is triggered to record data as the freshly mixed solution ages. Although older-style stopped-flow instruments employ air cylinders and stop syringes, KinTek uses numerically controlled servo motors for precise and reproducible rates of mixing. All KinTek stopped-flow models use this elegant and reliable syringe drive system affording a dead time of less than 1 ms.
Our instruments may cost a bit more than the cheaper air driven models, but they pay for themselves in terms of improved reliability, and the ease of use and sample economy means you get more results with less time and materials.
The Auto SF-120 offers outstanding sample economy, requiring only 120 microliters to load a new sample and collect data. The samples are automatically loaded using our proprietary computer-controlled syringe drive and syringe load valve. Simply pipette your sample into a small sample cup and press "Load". The automated loading and extraordinary sample economy allow you to collect more data in less time and using less of your precious samples. An optional robot allows unattended sample loading and data collection. Choose the Auto SF-120 if you need small sample volumes and/or have a large numbers of samples to test. More information...
The SF-300X requires only 300 microliters to load a new sample, second only to the Auto SF-120 in sample economy. What distinguishes the SF-300x is the ability to do efficient double mixing experiments and to add an equilibrium titration module. Choose the SF-300x if you need these capabilities or need to adapt the Stopped-flow to new detection methods. More information....
Chemical Quench-Flow Technology
Stopped-flow techniques are limited to the observation of an available optical signal for the reaction of interest and the optical signals cannot be interpreted rigorously if the extinction coefficients of intermediates or products are not known. Chemical quench-flow methods afford direct measurement of the conversion of substrates to products. By simultaneously fitting stopped-flow and chemical-quench-flow data using KinTek Explorer software, you can derive a single unique model to define the reaction mechanism. For example, see Dr. Johnson's work on HIV reverse transcriptase.
RQF-3 Rapid Chemical Quench Flow
The RQF-3 enables the mixing and quenching of reactants for times as short as 2 milliseconds, using reaction volumes as small as 15 microliters. Double Delay mixing is a standard feature. The RQF-3 also allows optional accessories for Rapid Freeze-Quench and Rapid Photolysis after mixing. Up to three mixing events can be performed. More information....